Application of CLARITY to Investigate the 3D Architecture of 14-3-3 Zeta Protein in AD

Honghong Song; Juan Feng; Jian Li; Yang Xia; Dezhong Yao

doi:doi:10.11648/j.ijbse.20170506.12

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Application of CLARITY to Investigate the 3D Architecture of 14-3-3 Zeta Protein in AD

Honghong Song, Juan Feng, Jian Li, Yang Xia, Dezhong Yao

Published in International Journal of Biomedical Science and Engineering (Volume 5, Issue 6)

Received: 5 December 2017 Published: 6 December 2017

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Abstract

CLARITY (Clear Lipid–exchanged Acrylamide–hybridized Rigid Imaging/ Immunostaining/ in situ–hybridization–compatible Tissue hydrogel) is a powerful, innovative, whole brain-clearing technology, and it has been successfully combined with the immunofluorescence staining to achieve the 3D visualization of some proteins or cells in mm-thick brain tissue or even the intact brains. These 3D information help to gain deeper understanding on the pathologic mechanism of some neuronal diseases (for example Parkinson and Alzheimer). 14-3-3 zeta is a highly-expressed protein in Alzheimer’s disease (AD) brain, which was closely related with the formation of Tau aggregation and neurofibrillary tangles. However, little useful information has been available concerning the 3D architecture of 14-3-3 zeta in AD disease. In this paper, the transgenic AD mice were used and the 1mm-thick brain slices were passively clarified. Immunofluorescence staining results showed that different from the control group, 14-3-3 zeta was mainly present around the hippocampus in AD mice. Additionally, the morphology of 14-3-3 zeta protein was filamentous with different lengths. This result will be helpful for exploring the in vivo role of 14-3-3 zeta protein during the progression of AD.

Published in	International Journal of Biomedical Science and Engineering (Volume 5, Issue 6)
DOI	10.11648/j.ijbse.20170506.12
Page(s)	68-71
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

AD, CLARITY, 14-3-Zeta Protein, Immunofluorescence

References

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APA Style

Honghong Song, Juan Feng, Jian Li, Yang Xia, Dezhong Yao. (2017). Application of CLARITY to Investigate the 3D Architecture of 14-3-3 Zeta Protein in AD. International Journal of Biomedical Science and Engineering, 5(6), 68-71. https://doi.org/10.11648/j.ijbse.20170506.12

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ACS Style

Honghong Song; Juan Feng; Jian Li; Yang Xia; Dezhong Yao. Application of CLARITY to Investigate the 3D Architecture of 14-3-3 Zeta Protein in AD. Int. J. Biomed. Sci. Eng. 2017, 5(6), 68-71. doi: 10.11648/j.ijbse.20170506.12

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AMA Style

Honghong Song, Juan Feng, Jian Li, Yang Xia, Dezhong Yao. Application of CLARITY to Investigate the 3D Architecture of 14-3-3 Zeta Protein in AD. Int J Biomed Sci Eng. 2017;5(6):68-71. doi: 10.11648/j.ijbse.20170506.12

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@article{10.11648/j.ijbse.20170506.12,
  author = {Honghong Song and Juan Feng and Jian Li and Yang Xia and Dezhong Yao},
  title = {Application of CLARITY to Investigate the 3D Architecture of 14-3-3 Zeta Protein in AD},
  journal = {International Journal of Biomedical Science and Engineering},
  volume = {5},
  number = {6},
  pages = {68-71},
  doi = {10.11648/j.ijbse.20170506.12},
  url = {https://doi.org/10.11648/j.ijbse.20170506.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijbse.20170506.12},
  abstract = {CLARITY (Clear Lipid–exchanged Acrylamide–hybridized Rigid Imaging/ Immunostaining/ in situ–hybridization–compatible Tissue hydrogel) is a powerful, innovative, whole brain-clearing technology, and it has been successfully combined with the immunofluorescence staining to achieve the 3D visualization of some proteins or cells in mm-thick brain tissue or even the intact brains. These 3D information help to gain deeper understanding on the pathologic mechanism of some neuronal diseases (for example Parkinson and Alzheimer). 14-3-3 zeta is a highly-expressed protein in Alzheimer’s disease (AD) brain, which was closely related with the formation of Tau aggregation and neurofibrillary tangles. However, little useful information has been available concerning the 3D architecture of 14-3-3 zeta in AD disease. In this paper, the transgenic AD mice were used and the 1mm-thick brain slices were passively clarified. Immunofluorescence staining results showed that different from the control group, 14-3-3 zeta was mainly present around the hippocampus in AD mice. Additionally, the morphology of 14-3-3 zeta protein was filamentous with different lengths. This result will be helpful for exploring the in vivo role of 14-3-3 zeta protein during the progression of AD.},
 year = {2017}
}

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TY  - JOUR
T1  - Application of CLARITY to Investigate the 3D Architecture of 14-3-3 Zeta Protein in AD
AU  - Honghong Song
AU  - Juan Feng
AU  - Jian Li
AU  - Yang Xia
AU  - Dezhong Yao
Y1  - 2017/12/06
PY  - 2017
N1  - https://doi.org/10.11648/j.ijbse.20170506.12
DO  - 10.11648/j.ijbse.20170506.12
T2  - International Journal of Biomedical Science and Engineering
JF  - International Journal of Biomedical Science and Engineering
JO  - International Journal of Biomedical Science and Engineering
SP  - 68
EP  - 71
PB  - Science Publishing Group
SN  - 2376-7235
UR  - https://doi.org/10.11648/j.ijbse.20170506.12
AB  - CLARITY (Clear Lipid–exchanged Acrylamide–hybridized Rigid Imaging/ Immunostaining/ in situ–hybridization–compatible Tissue hydrogel) is a powerful, innovative, whole brain-clearing technology, and it has been successfully combined with the immunofluorescence staining to achieve the 3D visualization of some proteins or cells in mm-thick brain tissue or even the intact brains. These 3D information help to gain deeper understanding on the pathologic mechanism of some neuronal diseases (for example Parkinson and Alzheimer). 14-3-3 zeta is a highly-expressed protein in Alzheimer’s disease (AD) brain, which was closely related with the formation of Tau aggregation and neurofibrillary tangles. However, little useful information has been available concerning the 3D architecture of 14-3-3 zeta in AD disease. In this paper, the transgenic AD mice were used and the 1mm-thick brain slices were passively clarified. Immunofluorescence staining results showed that different from the control group, 14-3-3 zeta was mainly present around the hippocampus in AD mice. Additionally, the morphology of 14-3-3 zeta protein was filamentous with different lengths. This result will be helpful for exploring the in vivo role of 14-3-3 zeta protein during the progression of AD.
VL  - 5
IS  - 6
ER  -

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Author Information

Honghong Song

College of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, China
Juan Feng

College of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, China
Jian Li

College of Life Science, Beijing University of Chinese Medicine, Beijing, China
Yang Xia

College of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, China
Dezhong Yao

College of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, China

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Plain Text BibTeX RIS

APA Style

Honghong Song, Juan Feng, Jian Li, Yang Xia, Dezhong Yao. (2017). Application of CLARITY to Investigate the 3D Architecture of 14-3-3 Zeta Protein in AD. International Journal of Biomedical Science and Engineering, 5(6), 68-71. https://doi.org/10.11648/j.ijbse.20170506.12

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ACS Style

Honghong Song; Juan Feng; Jian Li; Yang Xia; Dezhong Yao. Application of CLARITY to Investigate the 3D Architecture of 14-3-3 Zeta Protein in AD. Int. J. Biomed. Sci. Eng. 2017, 5(6), 68-71. doi: 10.11648/j.ijbse.20170506.12

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AMA Style

Honghong Song, Juan Feng, Jian Li, Yang Xia, Dezhong Yao. Application of CLARITY to Investigate the 3D Architecture of 14-3-3 Zeta Protein in AD. Int J Biomed Sci Eng. 2017;5(6):68-71. doi: 10.11648/j.ijbse.20170506.12

Copy | Download

@article{10.11648/j.ijbse.20170506.12,
  author = {Honghong Song and Juan Feng and Jian Li and Yang Xia and Dezhong Yao},
  title = {Application of CLARITY to Investigate the 3D Architecture of 14-3-3 Zeta Protein in AD},
  journal = {International Journal of Biomedical Science and Engineering},
  volume = {5},
  number = {6},
  pages = {68-71},
  doi = {10.11648/j.ijbse.20170506.12},
  url = {https://doi.org/10.11648/j.ijbse.20170506.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijbse.20170506.12},
  abstract = {CLARITY (Clear Lipid–exchanged Acrylamide–hybridized Rigid Imaging/ Immunostaining/ in situ–hybridization–compatible Tissue hydrogel) is a powerful, innovative, whole brain-clearing technology, and it has been successfully combined with the immunofluorescence staining to achieve the 3D visualization of some proteins or cells in mm-thick brain tissue or even the intact brains. These 3D information help to gain deeper understanding on the pathologic mechanism of some neuronal diseases (for example Parkinson and Alzheimer). 14-3-3 zeta is a highly-expressed protein in Alzheimer’s disease (AD) brain, which was closely related with the formation of Tau aggregation and neurofibrillary tangles. However, little useful information has been available concerning the 3D architecture of 14-3-3 zeta in AD disease. In this paper, the transgenic AD mice were used and the 1mm-thick brain slices were passively clarified. Immunofluorescence staining results showed that different from the control group, 14-3-3 zeta was mainly present around the hippocampus in AD mice. Additionally, the morphology of 14-3-3 zeta protein was filamentous with different lengths. This result will be helpful for exploring the in vivo role of 14-3-3 zeta protein during the progression of AD.},
 year = {2017}
}

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TY  - JOUR
T1  - Application of CLARITY to Investigate the 3D Architecture of 14-3-3 Zeta Protein in AD
AU  - Honghong Song
AU  - Juan Feng
AU  - Jian Li
AU  - Yang Xia
AU  - Dezhong Yao
Y1  - 2017/12/06
PY  - 2017
N1  - https://doi.org/10.11648/j.ijbse.20170506.12
DO  - 10.11648/j.ijbse.20170506.12
T2  - International Journal of Biomedical Science and Engineering
JF  - International Journal of Biomedical Science and Engineering
JO  - International Journal of Biomedical Science and Engineering
SP  - 68
EP  - 71
PB  - Science Publishing Group
SN  - 2376-7235
UR  - https://doi.org/10.11648/j.ijbse.20170506.12
AB  - CLARITY (Clear Lipid–exchanged Acrylamide–hybridized Rigid Imaging/ Immunostaining/ in situ–hybridization–compatible Tissue hydrogel) is a powerful, innovative, whole brain-clearing technology, and it has been successfully combined with the immunofluorescence staining to achieve the 3D visualization of some proteins or cells in mm-thick brain tissue or even the intact brains. These 3D information help to gain deeper understanding on the pathologic mechanism of some neuronal diseases (for example Parkinson and Alzheimer). 14-3-3 zeta is a highly-expressed protein in Alzheimer’s disease (AD) brain, which was closely related with the formation of Tau aggregation and neurofibrillary tangles. However, little useful information has been available concerning the 3D architecture of 14-3-3 zeta in AD disease. In this paper, the transgenic AD mice were used and the 1mm-thick brain slices were passively clarified. Immunofluorescence staining results showed that different from the control group, 14-3-3 zeta was mainly present around the hippocampus in AD mice. Additionally, the morphology of 14-3-3 zeta protein was filamentous with different lengths. This result will be helpful for exploring the in vivo role of 14-3-3 zeta protein during the progression of AD.
VL  - 5
IS  - 6
ER  -

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