Effect of High Pressure Homogenization on Aqueous Phase Solvent Extraction of Lipids from Nannochloris Oculata Microalgae

Nalin Samarasinghe; Sandun Fernando; Brock Faulkner

doi:doi:10.11648/j.jenr.20120101.11

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Effect of High Pressure Homogenization on Aqueous Phase Solvent Extraction of Lipids from Nannochloris Oculata Microalgae

Nalin Samarasinghe, Sandun Fernando, Brock Faulkner

Published in Journal of Energy and Natural Resources (Volume 1, Issue 1)

Published: 30 December 2012

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Abstract

The ability to extract lipids from high-moisture Nannochloris Oculata algal biomass disrupted with high pressure homogenization was investigated. During the first phase, the effect of high pressure homogenization (system pressure and number of passes) on disrupting aqueous algae (of different concentrations and degree of stress) was investigated. Se-condly, the effect of degree of cell wall disruption on the amount of lipids extracted with three solvents, namely: hexane, dichloromethane and chloroform, were compared. Studies reveled that high pressure homogenization is effective on cell disruption while the amount of system pressure being the most significant factor affecting the degree of cell breakage. Al-though the number of passes had some impact, the level of disruption seemed to level-off after a certain number of passes. The study revealed that slightly polar solvents (such as chloroform and dichloromethane) performed better in aqueous-phase lipid extractions as compared to hexane. Also, it was revealed that it was not necessary to disrupt the algal cells completely to achieve appreciable levels of lipid yields. In fact, conditions that exerted only 20% of the cells to completely disrupt, allowed sufficient damage to liberate most of the lipids contained in the remainder of the cells.

Published in	Journal of Energy and Natural Resources (Volume 1, Issue 1)
DOI	10.11648/j.jenr.20120101.11
Page(s)	1-7
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2012. Published by Science Publishing Group

Keywords

Microalgae, Homogenization, Extraction, Lipids

References

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APA Style

Nalin Samarasinghe, Sandun Fernando, Brock Faulkner. (2012). Effect of High Pressure Homogenization on Aqueous Phase Solvent Extraction of Lipids from Nannochloris Oculata Microalgae. Journal of Energy and Natural Resources, 1(1), 1-7. https://doi.org/10.11648/j.jenr.20120101.11

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ACS Style

Nalin Samarasinghe; Sandun Fernando; Brock Faulkner. Effect of High Pressure Homogenization on Aqueous Phase Solvent Extraction of Lipids from Nannochloris Oculata Microalgae. J. Energy Nat. Resour. 2012, 1(1), 1-7. doi: 10.11648/j.jenr.20120101.11

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AMA Style

Nalin Samarasinghe, Sandun Fernando, Brock Faulkner. Effect of High Pressure Homogenization on Aqueous Phase Solvent Extraction of Lipids from Nannochloris Oculata Microalgae. J Energy Nat Resour. 2012;1(1):1-7. doi: 10.11648/j.jenr.20120101.11

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@article{10.11648/j.jenr.20120101.11,
  author = {Nalin Samarasinghe and Sandun Fernando and Brock Faulkner},
  title = {Effect of High Pressure Homogenization on Aqueous Phase Solvent Extraction of Lipids from Nannochloris Oculata Microalgae},
  journal = {Journal of Energy and Natural Resources},
  volume = {1},
  number = {1},
  pages = {1-7},
  doi = {10.11648/j.jenr.20120101.11},
  url = {https://doi.org/10.11648/j.jenr.20120101.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jenr.20120101.11},
  abstract = {The ability to extract lipids from high-moisture Nannochloris Oculata algal biomass disrupted with high pressure homogenization was investigated. During the first phase, the effect of high pressure homogenization (system pressure and number of passes) on disrupting aqueous algae (of different concentrations and degree of stress) was investigated. Se-condly, the effect of degree of cell wall disruption on the amount of lipids extracted with three solvents, namely: hexane, dichloromethane and chloroform, were compared. Studies reveled that high pressure homogenization is effective on cell disruption while the amount of system pressure being the most significant factor affecting the degree of cell breakage. Al-though the number of passes had some impact, the level of disruption seemed to level-off after a certain number of passes. The study revealed that slightly polar solvents (such as chloroform and dichloromethane) performed better in aqueous-phase lipid extractions as compared to hexane. Also, it was revealed that it was not necessary to disrupt the algal cells completely to achieve appreciable levels of lipid yields. In fact, conditions that exerted only 20% of the cells to completely disrupt, allowed sufficient damage to liberate most of the lipids contained in the remainder of the cells.},
 year = {2012}
}

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TY  - JOUR
T1  - Effect of High Pressure Homogenization on Aqueous Phase Solvent Extraction of Lipids from Nannochloris Oculata Microalgae
AU  - Nalin Samarasinghe
AU  - Sandun Fernando
AU  - Brock Faulkner
Y1  - 2012/12/30
PY  - 2012
N1  - https://doi.org/10.11648/j.jenr.20120101.11
DO  - 10.11648/j.jenr.20120101.11
T2  - Journal of Energy and Natural Resources
JF  - Journal of Energy and Natural Resources
JO  - Journal of Energy and Natural Resources
SP  - 1
EP  - 7
PB  - Science Publishing Group
SN  - 2330-7404
UR  - https://doi.org/10.11648/j.jenr.20120101.11
AB  - The ability to extract lipids from high-moisture Nannochloris Oculata algal biomass disrupted with high pressure homogenization was investigated. During the first phase, the effect of high pressure homogenization (system pressure and number of passes) on disrupting aqueous algae (of different concentrations and degree of stress) was investigated. Se-condly, the effect of degree of cell wall disruption on the amount of lipids extracted with three solvents, namely: hexane, dichloromethane and chloroform, were compared. Studies reveled that high pressure homogenization is effective on cell disruption while the amount of system pressure being the most significant factor affecting the degree of cell breakage. Al-though the number of passes had some impact, the level of disruption seemed to level-off after a certain number of passes. The study revealed that slightly polar solvents (such as chloroform and dichloromethane) performed better in aqueous-phase lipid extractions as compared to hexane. Also, it was revealed that it was not necessary to disrupt the algal cells completely to achieve appreciable levels of lipid yields. In fact, conditions that exerted only 20% of the cells to completely disrupt, allowed sufficient damage to liberate most of the lipids contained in the remainder of the cells.
VL  - 1
IS  - 1
ER  -

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Author Information

Nalin Samarasinghe

Department of Biological and Agricultural Engineering, Scoates Hall, TAMU, Texas A&M University, College Station TX USA
Sandun Fernando

Department of Biological and Agricultural Engineering, Scoates Hall, TAMU, Texas A&M University, College Station TX USA
Brock Faulkner

Department of Biological and Agricultural Engineering, Scoates Hall, TAMU, Texas A&M University, College Station TX USA

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APA Style

Nalin Samarasinghe, Sandun Fernando, Brock Faulkner. (2012). Effect of High Pressure Homogenization on Aqueous Phase Solvent Extraction of Lipids from Nannochloris Oculata Microalgae. Journal of Energy and Natural Resources, 1(1), 1-7. https://doi.org/10.11648/j.jenr.20120101.11

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ACS Style

Nalin Samarasinghe; Sandun Fernando; Brock Faulkner. Effect of High Pressure Homogenization on Aqueous Phase Solvent Extraction of Lipids from Nannochloris Oculata Microalgae. J. Energy Nat. Resour. 2012, 1(1), 1-7. doi: 10.11648/j.jenr.20120101.11

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AMA Style

Nalin Samarasinghe, Sandun Fernando, Brock Faulkner. Effect of High Pressure Homogenization on Aqueous Phase Solvent Extraction of Lipids from Nannochloris Oculata Microalgae. J Energy Nat Resour. 2012;1(1):1-7. doi: 10.11648/j.jenr.20120101.11

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@article{10.11648/j.jenr.20120101.11,
  author = {Nalin Samarasinghe and Sandun Fernando and Brock Faulkner},
  title = {Effect of High Pressure Homogenization on Aqueous Phase Solvent Extraction of Lipids from Nannochloris Oculata Microalgae},
  journal = {Journal of Energy and Natural Resources},
  volume = {1},
  number = {1},
  pages = {1-7},
  doi = {10.11648/j.jenr.20120101.11},
  url = {https://doi.org/10.11648/j.jenr.20120101.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jenr.20120101.11},
  abstract = {The ability to extract lipids from high-moisture Nannochloris Oculata algal biomass disrupted with high pressure homogenization was investigated. During the first phase, the effect of high pressure homogenization (system pressure and number of passes) on disrupting aqueous algae (of different concentrations and degree of stress) was investigated. Se-condly, the effect of degree of cell wall disruption on the amount of lipids extracted with three solvents, namely: hexane, dichloromethane and chloroform, were compared. Studies reveled that high pressure homogenization is effective on cell disruption while the amount of system pressure being the most significant factor affecting the degree of cell breakage. Al-though the number of passes had some impact, the level of disruption seemed to level-off after a certain number of passes. The study revealed that slightly polar solvents (such as chloroform and dichloromethane) performed better in aqueous-phase lipid extractions as compared to hexane. Also, it was revealed that it was not necessary to disrupt the algal cells completely to achieve appreciable levels of lipid yields. In fact, conditions that exerted only 20% of the cells to completely disrupt, allowed sufficient damage to liberate most of the lipids contained in the remainder of the cells.},
 year = {2012}
}

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TY  - JOUR
T1  - Effect of High Pressure Homogenization on Aqueous Phase Solvent Extraction of Lipids from Nannochloris Oculata Microalgae
AU  - Nalin Samarasinghe
AU  - Sandun Fernando
AU  - Brock Faulkner
Y1  - 2012/12/30
PY  - 2012
N1  - https://doi.org/10.11648/j.jenr.20120101.11
DO  - 10.11648/j.jenr.20120101.11
T2  - Journal of Energy and Natural Resources
JF  - Journal of Energy and Natural Resources
JO  - Journal of Energy and Natural Resources
SP  - 1
EP  - 7
PB  - Science Publishing Group
SN  - 2330-7404
UR  - https://doi.org/10.11648/j.jenr.20120101.11
AB  - The ability to extract lipids from high-moisture Nannochloris Oculata algal biomass disrupted with high pressure homogenization was investigated. During the first phase, the effect of high pressure homogenization (system pressure and number of passes) on disrupting aqueous algae (of different concentrations and degree of stress) was investigated. Se-condly, the effect of degree of cell wall disruption on the amount of lipids extracted with three solvents, namely: hexane, dichloromethane and chloroform, were compared. Studies reveled that high pressure homogenization is effective on cell disruption while the amount of system pressure being the most significant factor affecting the degree of cell breakage. Al-though the number of passes had some impact, the level of disruption seemed to level-off after a certain number of passes. The study revealed that slightly polar solvents (such as chloroform and dichloromethane) performed better in aqueous-phase lipid extractions as compared to hexane. Also, it was revealed that it was not necessary to disrupt the algal cells completely to achieve appreciable levels of lipid yields. In fact, conditions that exerted only 20% of the cells to completely disrupt, allowed sufficient damage to liberate most of the lipids contained in the remainder of the cells.
VL  - 1
IS  - 1
ER  -

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