The invasion and metastasis are linked to the rapid progression and poor prognosis of hepatocarcinoma patients. Lymphatic metastasis is potentially involved in above pathogenesis with unclear mechanism. Previously, we found the deregulation of annexin A5 (ANXA5), a member of Ca2+-regulated phospholipid- and membrane-binding annexin family protein, mediated the in vivo malignancy, lymph node metastasis (LNM) rate and level of mice transplanted with Hca-P, a murine hepatocarcinoma cell line with the LNM potential rate of ~25%. Current work aimed to investigate the influence with action mechanism of ANXA5 overexpression on the in vitro malignant behaviours of Hca-P cells. For overexpressing ANXA5, the Anxa5 gene was ligated into pCDNA3.1 (+) vector and transfected into Hca-P named as Hca-P-ANXA5up. Hca-P transfected with empty pCDNA3.1 (+) vector was named as Hca-P-mock and used as the control. The monoclonal Hca-P-mock and Hca-P-ANXA5up cell lines were obtained against G418 screening using limiting dilution method. Compared with the Hca-P-mock cells, Western blotting assay indicated ANXA5 expression level was increased by 50.1% (p=0.025) in the Hca-P-ANXA5up cells. Transwell chamber assays indicated that the migration and invasion capacities of Hca-P-ANXA5up cells were increased by 150.2% (p=0.001) and 94.8% (p=0.003) than Hca-P-mock cells. ANXA5 overexpression enhanced the levels of p-MEK (Ser217/221), ERK1, ERK2, p-ERK1 (Thr202/Tyr204) and p-ERK2 (Thr185/Tyr187), and suppressed the levels of E-Cadherin, Snail and Slug in Hca-P cells. Current work shows ANXA5 overexpression enhances the malignant behaviours of hepatocarcinoma Hca-P cells through activating p-MEK-ERK pathway and suppressing E-Cadherin, Snail and Slug. It is of potential value in tumor malignancy and lymphatic metastasis of hepatocarcinoma.
| Published in | American Journal of Life Sciences (Volume 7, Issue 1) |
| DOI | 10.11648/j.ajls.20190701.16 |
| Page(s) | 31-37 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2019. Published by Science Publishing Group |
Hepatocarcinoma, ANXA5, ERK, E-Cadherin
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APA Style
Shuqing Liu, Chen Chen, Mingzhong Sun. (2019). ANXA5 Enhances Malignancy of Murine Hepatocarcinoma Hca-P Cells via ERK Activation and E-cadherin Suppression. American Journal of Life Sciences, 7(1), 31-37. https://doi.org/10.11648/j.ajls.20190701.16
ACS Style
Shuqing Liu; Chen Chen; Mingzhong Sun. ANXA5 Enhances Malignancy of Murine Hepatocarcinoma Hca-P Cells via ERK Activation and E-cadherin Suppression. Am. J. Life Sci. 2019, 7(1), 31-37. doi: 10.11648/j.ajls.20190701.16
AMA Style
Shuqing Liu, Chen Chen, Mingzhong Sun. ANXA5 Enhances Malignancy of Murine Hepatocarcinoma Hca-P Cells via ERK Activation and E-cadherin Suppression. Am J Life Sci. 2019;7(1):31-37. doi: 10.11648/j.ajls.20190701.16
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Download@article{10.11648/j.ajls.20190701.16,
author = {Shuqing Liu and Chen Chen and Mingzhong Sun},
title = {ANXA5 Enhances Malignancy of Murine Hepatocarcinoma Hca-P Cells via ERK Activation and E-cadherin Suppression},
journal = {American Journal of Life Sciences},
volume = {7},
number = {1},
pages = {31-37},
doi = {10.11648/j.ajls.20190701.16},
url = {https://doi.org/10.11648/j.ajls.20190701.16},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20190701.16},
abstract = {The invasion and metastasis are linked to the rapid progression and poor prognosis of hepatocarcinoma patients. Lymphatic metastasis is potentially involved in above pathogenesis with unclear mechanism. Previously, we found the deregulation of annexin A5 (ANXA5), a member of Ca2+-regulated phospholipid- and membrane-binding annexin family protein, mediated the in vivo malignancy, lymph node metastasis (LNM) rate and level of mice transplanted with Hca-P, a murine hepatocarcinoma cell line with the LNM potential rate of ~25%. Current work aimed to investigate the influence with action mechanism of ANXA5 overexpression on the in vitro malignant behaviours of Hca-P cells. For overexpressing ANXA5, the Anxa5 gene was ligated into pCDNA3.1 (+) vector and transfected into Hca-P named as Hca-P-ANXA5up. Hca-P transfected with empty pCDNA3.1 (+) vector was named as Hca-P-mock and used as the control. The monoclonal Hca-P-mock and Hca-P-ANXA5up cell lines were obtained against G418 screening using limiting dilution method. Compared with the Hca-P-mock cells, Western blotting assay indicated ANXA5 expression level was increased by 50.1% (p=0.025) in the Hca-P-ANXA5up cells. Transwell chamber assays indicated that the migration and invasion capacities of Hca-P-ANXA5up cells were increased by 150.2% (p=0.001) and 94.8% (p=0.003) than Hca-P-mock cells. ANXA5 overexpression enhanced the levels of p-MEK (Ser217/221), ERK1, ERK2, p-ERK1 (Thr202/Tyr204) and p-ERK2 (Thr185/Tyr187), and suppressed the levels of E-Cadherin, Snail and Slug in Hca-P cells. Current work shows ANXA5 overexpression enhances the malignant behaviours of hepatocarcinoma Hca-P cells through activating p-MEK-ERK pathway and suppressing E-Cadherin, Snail and Slug. It is of potential value in tumor malignancy and lymphatic metastasis of hepatocarcinoma.},
year = {2019}
}
TY - JOUR T1 - ANXA5 Enhances Malignancy of Murine Hepatocarcinoma Hca-P Cells via ERK Activation and E-cadherin Suppression AU - Shuqing Liu AU - Chen Chen AU - Mingzhong Sun Y1 - 2019/05/23 PY - 2019 N1 - https://doi.org/10.11648/j.ajls.20190701.16 DO - 10.11648/j.ajls.20190701.16 T2 - American Journal of Life Sciences JF - American Journal of Life Sciences JO - American Journal of Life Sciences SP - 31 EP - 37 PB - Science Publishing Group SN - 2328-5737 UR - https://doi.org/10.11648/j.ajls.20190701.16 AB - The invasion and metastasis are linked to the rapid progression and poor prognosis of hepatocarcinoma patients. Lymphatic metastasis is potentially involved in above pathogenesis with unclear mechanism. Previously, we found the deregulation of annexin A5 (ANXA5), a member of Ca2+-regulated phospholipid- and membrane-binding annexin family protein, mediated the in vivo malignancy, lymph node metastasis (LNM) rate and level of mice transplanted with Hca-P, a murine hepatocarcinoma cell line with the LNM potential rate of ~25%. Current work aimed to investigate the influence with action mechanism of ANXA5 overexpression on the in vitro malignant behaviours of Hca-P cells. For overexpressing ANXA5, the Anxa5 gene was ligated into pCDNA3.1 (+) vector and transfected into Hca-P named as Hca-P-ANXA5up. Hca-P transfected with empty pCDNA3.1 (+) vector was named as Hca-P-mock and used as the control. The monoclonal Hca-P-mock and Hca-P-ANXA5up cell lines were obtained against G418 screening using limiting dilution method. Compared with the Hca-P-mock cells, Western blotting assay indicated ANXA5 expression level was increased by 50.1% (p=0.025) in the Hca-P-ANXA5up cells. Transwell chamber assays indicated that the migration and invasion capacities of Hca-P-ANXA5up cells were increased by 150.2% (p=0.001) and 94.8% (p=0.003) than Hca-P-mock cells. ANXA5 overexpression enhanced the levels of p-MEK (Ser217/221), ERK1, ERK2, p-ERK1 (Thr202/Tyr204) and p-ERK2 (Thr185/Tyr187), and suppressed the levels of E-Cadherin, Snail and Slug in Hca-P cells. Current work shows ANXA5 overexpression enhances the malignant behaviours of hepatocarcinoma Hca-P cells through activating p-MEK-ERK pathway and suppressing E-Cadherin, Snail and Slug. It is of potential value in tumor malignancy and lymphatic metastasis of hepatocarcinoma. VL - 7 IS - 1 ER -
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