Expression of O6 Methyl Guanine Methyl Transferase (Mgmt) in Oral Squamous Cell Carcinoma Patients in Alexandria, Egypt (Smokers and Non-Smokers)

Hend Mohamed Helmy; Taissir Ali Omar; Sahar Mohamed ElSheikh; Ahmed Serag Habib

doi:doi:10.11648/j.ajls.20140204.15

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Expression of O6 Methyl Guanine Methyl Transferase (Mgmt) in Oral Squamous Cell Carcinoma Patients in Alexandria, Egypt (Smokers and Non-Smokers)

Hend Mohamed Helmy, Taissir Ali Omar, Sahar Mohamed ElSheikh, Ahmed Serag Habib

Published in American Journal of Life Sciences (Volume 2, Issue 4)

Received: 6 August 2014 Accepted: 16 August 2014 Published: 30 August 2014

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Abstract

Objectives: O6-methylguanine-DNA methyltransferase (MGMT) removes mutagenic, carcinogenic, and cytotoxic adducts from O6-methylguanine in DNA through a direct reversal mechanism. Decreased expression of MGMT has been reported in a variety of human malignant tumors. The purpose of this study was to clarify the correlation of MGMT expression levels in oral squamous cell carcinoma (OSCC) and cigarette smoking. Study Design: MGMT protein expression in 22 cases of oral squamous cell carcinoma by immunohistochemistry was investigated. Correlation with detailed tobacco history was then tested by statistical analysis. Results: All the non smoker OSCC cases showed positive immunoexpression. However 3 cases of the smoker patient revealed a weak positive immunostaining with only sporadic cells at the periphery. Conclusion: The results suggest that the absence of MGMT expression is related strongly to tobacco smoking and, thus, might be a significant event in oral carcinogenesis.

Published in	American Journal of Life Sciences (Volume 2, Issue 4)
DOI	10.11648/j.ajls.20140204.15
Page(s)	217-223
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2014. Published by Science Publishing Group

Keywords

O6-Methylguanine-DNA Methyltransferase, Tobacco, Oral Squamous Cell Carcinoma

References

[1]	Jemal A, Siegel R, Ward E, Hao Y, Xu J, Thun MJ. Cancer statistics, 2009. CA Cancer J Clin. 2009 Jul-Aug;59(4):225-49.
[2]	Jones D, Rankin K. Oral Cancer and Associated Risk Factors. In: AA B, editor. Prevention in Clinical Oral Health Care. St Louis: Mosby Yearbook; 2008.
[3]	Chen KM, Guttenplan JB, Zhang SM, Aliaga C, Cooper TK, Sun YW, et al. Mechanisms of oral carcinogenesis induced by dibenzo[a,l]pyrene: an environmental pollutant and a tobacco smoke constituent. Int J Cancer. Sep 15;133(6):1300-9.
[4]	Gerson SL. MGMT: its role in cancer aetiology and cancer therapeutics. Nat Rev Cancer. 2004 Apr;4(4):296-307.
[5]	Kaina B, Christmann M, Naumann S, Roos WP. MGMT: key node in the battle against genotoxicity, carcinogenicity and apoptosis induced by alkylating agents. DNA Repair (Amst). 2007 Aug 1;6(8):1079-99.
[6]	Pegg AE, Fang Q, Loktionova NA. Human variants of O6-alkylguanine-DNA alkyltransferase. DNA Repair (Amst). 2007 Aug 1;6(8):1071-8.
[7]	Soejima H, Zhao W, Mukai T. Epigenetic silencing of the MGMT gene in cancer. Biochem Cell Biol. 2005 Aug;83(4):429-37.
[8]	Christmann M, Kaina B. O(6)-methylguanine-DNA methyltransferase (MGMT): impact on cancer risk in response to tobacco smoke. Mutat Res. Aug 1;736(1-2):64-74.
[9]	Neto JC, Ikoma MM, Carvalho KC, Vassallo J, De Brot M, Gobbi H, et al. MGMT and PTEN as potential prognostic markers in breast cancer. Exp Mol Pathol. Feb;92(1):20-6.
[10]	Nozoe T, Korenaga D, Kabashima A, Sugimachi K. Smoking-related increase of O(6)-methylguanine-DNA methyltransferase expression in squamous cell carcinoma of the esophagus. Cancer Lett. 2002 Oct 8;184(1):49-55.
[11]	Kreth S, Thon N, Eigenbrod S, Lutz J, Ledderose C, Egensperger R, et al. O-methylguanine-DNA methyltransferase (MGMT) mRNA expression predicts outcome in malignant glioma independent of MGMT promoter methylation. PLoS One.6(2):e17156.
[12]	Giaginis C, Michailidi C, Stolakis V, Alexandrou P, Tsourouflis G, Klijanienko J, et al. Expression of DNA repair proteins MSH2, MLH1 and MGMT in human benign and malignant thyroid lesions: an immunohistochemical study. Med Sci Monit. Feb 25;17(3):BR81-90.
[13]	Sawhney M, Rohatgi N, Kaur J, Gupta SD, Deo SV, Shukla NK, et al. MGMT expression in oral precancerous and cancerous lesions: correlation with progression, nodal metastasis and poor prognosis. Oral Oncol. 2007 May;43(5):515-22.
[14]	Huang SH, Lee HS, Mar K, Ji DD, Huang MS, Hsia KT. Loss expression of O6-methylguanine DNA methyltransferase by promoter hypermethylation and its relationship to betel quid chewing in oral squamous cell carcinoma. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. Jun;109(6):883-9.
[15]	Lee SS, Tsai CH, Yu CC, Ho YC, Hsu HI, Chang YC. The expression of O(6) -methylguanine-DNA methyltransferase in human oral keratinocytes stimulated with arecoline. J Oral Pathol Med. Sep;42(8):600-5.
[16]	Peterson CL, Cote J. Cellular machineries for chromosomal DNA repair. Genes Dev. 2004 Mar 15;18(6):602-16.
[17]	Hsieh P. Molecular mechanisms of DNA mismatch repair. Mutat Res. 2001 Jul 12;486(2):71-87.
[18]	Zuo C, Ai L, Ratliff P, Suen JY, Hanna E, Brent TP, et al. O6-methylguanine-DNA methyltransferase gene: epigenetic silencing and prognostic value in head and neck squamous cell carcinoma. Cancer Epidemiol Biomarkers Prev. 2004 Jun;13(6):967-75.
[19]	Chen JM, Zhang YP, Wang C, Sun Y, Fujimoto J, Ikenaga M. O6-methylguanine-DNA methyltransferase activity in human tumors. Carcinogenesis. 1992 Sep;13(9):1503-7.
[20]	Rodriguez MJ, Acha A, Ruesga MT, Rodriguez C, Rivera JM, Aguirre JM. Loss of expression of DNA repair enzyme MGMT in oral leukoplakia and early oral squamous cell carcinoma. A prognostic tool? Cancer Lett. 2007 Jan 8;245(1-2):263-8.
[21]	2009 WHOROftEM. Global Adult Tobacco Survey: Egypt Country Report. egypt: WHO; 2012 may Contract No.: Document Number\|.
[22]	Mitra S. MGMT: a personal perspective. DNA Repair (Amst). 2007 Aug 1;6(8):1064-70.
[23]	Betel-quid and areca-nut chewing and some areca-nut derived nitrosamines. IARC Monogr Eval Carcinog Risks Hum. 2004;85:1-334.
[24]	Leffondre K, Abrahamowicz M, Siemiatycki J, Rachet B. Modeling smoking history: a comparison of different approaches. Am J Epidemiol. 2002 Nov 1;156(9):813-23.
[25]	Lagergren J, Bergstrom R, Lindgren A, Nyren O. The role of tobacco, snuff and alcohol use in the aetiology of cancer of the oesophagus and gastric cardia. Int J Cancer. 2000 Feb 1;85(3):340-6.
[26]	Wu AH, Wan P, Bernstein L. A multiethnic population-based study of smoking, alcohol and body size and risk of adenocarcinomas of the stomach and esophagus (United States). Cancer Causes Control. 2001 Oct;12(8):721-32.
[27]	Pandeya N, Williams GM, Sadhegi S, Green AC, Webb PM, Whiteman DC. Associations of duration, intensity, and quantity of smoking with adenocarcinoma and squamous cell carcinoma of the esophagus. Am J Epidemiol. 2008 Jul 1;168(1):105-14.
[28]	Freedman ND, Abnet CC, Leitzmann MF, Mouw T, Subar AF, Hollenbeck AR, et al. A prospective study of tobacco, alcohol, and the risk of esophageal and gastric cancer subtypes. Am J Epidemiol. 2007 Jun 15;165(12):1424-33.
[29]	Esteller M. Aberrant DNA methylation as a cancer-inducing mechanism. Annu Rev Pharmacol Toxicol. 2005;45:629-56.
[30]	Matsukura S, Miyazaki K, Yakushiji H, Ogawa A, Harimaya K, Nakabeppu Y, et al. Expression and prognostic significance of O6-methylguanine-DNA methyltransferase in hepatocellular, gastric, and breast cancers. Ann Surg Oncol. 2001 Dec;8(10):807-16.
[31]	Komine C, Watanabe T, Katayama Y, Yoshino A, Yokoyama T, Fukushima T. Promoter hypermethylation of the DNA repair gene O6-methylguanine-DNA methyltransferase is an independent predictor of shortened progression free survival in patients with low-grade diffuse astrocytomas. Brain Pathol. 2003 Apr;13(2):176-84.

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APA Style

Hend Mohamed Helmy, Taissir Ali Omar, Sahar Mohamed ElSheikh, Ahmed Serag Habib. (2014). Expression of O6 Methyl Guanine Methyl Transferase (Mgmt) in Oral Squamous Cell Carcinoma Patients in Alexandria, Egypt (Smokers and Non-Smokers). American Journal of Life Sciences, 2(4), 217-223. https://doi.org/10.11648/j.ajls.20140204.15

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ACS Style

Hend Mohamed Helmy; Taissir Ali Omar; Sahar Mohamed ElSheikh; Ahmed Serag Habib. Expression of O6 Methyl Guanine Methyl Transferase (Mgmt) in Oral Squamous Cell Carcinoma Patients in Alexandria, Egypt (Smokers and Non-Smokers). Am. J. Life Sci. 2014, 2(4), 217-223. doi: 10.11648/j.ajls.20140204.15

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AMA Style

Hend Mohamed Helmy, Taissir Ali Omar, Sahar Mohamed ElSheikh, Ahmed Serag Habib. Expression of O6 Methyl Guanine Methyl Transferase (Mgmt) in Oral Squamous Cell Carcinoma Patients in Alexandria, Egypt (Smokers and Non-Smokers). Am J Life Sci. 2014;2(4):217-223. doi: 10.11648/j.ajls.20140204.15

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@article{10.11648/j.ajls.20140204.15,
  author = {Hend Mohamed Helmy and Taissir Ali Omar and Sahar Mohamed ElSheikh and Ahmed Serag Habib},
  title = {Expression of O6 Methyl Guanine Methyl Transferase (Mgmt) in Oral Squamous Cell Carcinoma Patients in Alexandria, Egypt (Smokers and Non-Smokers)},
  journal = {American Journal of Life Sciences},
  volume = {2},
  number = {4},
  pages = {217-223},
  doi = {10.11648/j.ajls.20140204.15},
  url = {https://doi.org/10.11648/j.ajls.20140204.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20140204.15},
  abstract = {Objectives: O6-methylguanine-DNA methyltransferase (MGMT) removes mutagenic, carcinogenic, and cytotoxic adducts from O6-methylguanine in DNA through a direct reversal mechanism. Decreased expression of MGMT has been reported in a variety of human malignant tumors. The purpose of this study was to clarify the correlation of MGMT expression levels in oral squamous cell carcinoma (OSCC) and cigarette smoking. Study Design: MGMT protein expression in 22 cases of oral squamous cell carcinoma by immunohistochemistry was investigated. Correlation with detailed tobacco history was then tested by statistical analysis. Results: All the non smoker OSCC cases showed positive immunoexpression. However 3 cases of the smoker patient revealed a weak positive immunostaining with only sporadic cells at the periphery. Conclusion: The results suggest that the absence of MGMT expression is related strongly to tobacco smoking and, thus, might be a significant event in oral carcinogenesis.},
 year = {2014}
}

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TY  - JOUR
T1  - Expression of O6 Methyl Guanine Methyl Transferase (Mgmt) in Oral Squamous Cell Carcinoma Patients in Alexandria, Egypt (Smokers and Non-Smokers)
AU  - Hend Mohamed Helmy
AU  - Taissir Ali Omar
AU  - Sahar Mohamed ElSheikh
AU  - Ahmed Serag Habib
Y1  - 2014/08/30
PY  - 2014
N1  - https://doi.org/10.11648/j.ajls.20140204.15
DO  - 10.11648/j.ajls.20140204.15
T2  - American Journal of Life Sciences
JF  - American Journal of Life Sciences
JO  - American Journal of Life Sciences
SP  - 217
EP  - 223
PB  - Science Publishing Group
SN  - 2328-5737
UR  - https://doi.org/10.11648/j.ajls.20140204.15
AB  - Objectives: O6-methylguanine-DNA methyltransferase (MGMT) removes mutagenic, carcinogenic, and cytotoxic adducts from O6-methylguanine in DNA through a direct reversal mechanism. Decreased expression of MGMT has been reported in a variety of human malignant tumors. The purpose of this study was to clarify the correlation of MGMT expression levels in oral squamous cell carcinoma (OSCC) and cigarette smoking. Study Design: MGMT protein expression in 22 cases of oral squamous cell carcinoma by immunohistochemistry was investigated. Correlation with detailed tobacco history was then tested by statistical analysis. Results: All the non smoker OSCC cases showed positive immunoexpression. However 3 cases of the smoker patient revealed a weak positive immunostaining with only sporadic cells at the periphery. Conclusion: The results suggest that the absence of MGMT expression is related strongly to tobacco smoking and, thus, might be a significant event in oral carcinogenesis.
VL  - 2
IS  - 4
ER  -

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Author Information

Hend Mohamed Helmy

Department of Oral Pathology, Faculty of Dentistry, Alexandria University, Egypt
Taissir Ali Omar

Department of Oral Pathology, Faculty of Dentistry, Alexandria University, Egypt
Sahar Mohamed ElSheikh

Department of Oral Pathology, Faculty of Dentistry, Alexandria University, Egypt
Ahmed Serag Habib

Department of Cranio Maxillofacial and Plastic Surgery, Faculty of Dentistry, Alexandria University, Egypt

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Plain Text BibTeX RIS

APA Style

Hend Mohamed Helmy, Taissir Ali Omar, Sahar Mohamed ElSheikh, Ahmed Serag Habib. (2014). Expression of O6 Methyl Guanine Methyl Transferase (Mgmt) in Oral Squamous Cell Carcinoma Patients in Alexandria, Egypt (Smokers and Non-Smokers). American Journal of Life Sciences, 2(4), 217-223. https://doi.org/10.11648/j.ajls.20140204.15

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ACS Style

Hend Mohamed Helmy; Taissir Ali Omar; Sahar Mohamed ElSheikh; Ahmed Serag Habib. Expression of O6 Methyl Guanine Methyl Transferase (Mgmt) in Oral Squamous Cell Carcinoma Patients in Alexandria, Egypt (Smokers and Non-Smokers). Am. J. Life Sci. 2014, 2(4), 217-223. doi: 10.11648/j.ajls.20140204.15

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AMA Style

Hend Mohamed Helmy, Taissir Ali Omar, Sahar Mohamed ElSheikh, Ahmed Serag Habib. Expression of O6 Methyl Guanine Methyl Transferase (Mgmt) in Oral Squamous Cell Carcinoma Patients in Alexandria, Egypt (Smokers and Non-Smokers). Am J Life Sci. 2014;2(4):217-223. doi: 10.11648/j.ajls.20140204.15

Copy | Download

@article{10.11648/j.ajls.20140204.15,
  author = {Hend Mohamed Helmy and Taissir Ali Omar and Sahar Mohamed ElSheikh and Ahmed Serag Habib},
  title = {Expression of O6 Methyl Guanine Methyl Transferase (Mgmt) in Oral Squamous Cell Carcinoma Patients in Alexandria, Egypt (Smokers and Non-Smokers)},
  journal = {American Journal of Life Sciences},
  volume = {2},
  number = {4},
  pages = {217-223},
  doi = {10.11648/j.ajls.20140204.15},
  url = {https://doi.org/10.11648/j.ajls.20140204.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20140204.15},
  abstract = {Objectives: O6-methylguanine-DNA methyltransferase (MGMT) removes mutagenic, carcinogenic, and cytotoxic adducts from O6-methylguanine in DNA through a direct reversal mechanism. Decreased expression of MGMT has been reported in a variety of human malignant tumors. The purpose of this study was to clarify the correlation of MGMT expression levels in oral squamous cell carcinoma (OSCC) and cigarette smoking. Study Design: MGMT protein expression in 22 cases of oral squamous cell carcinoma by immunohistochemistry was investigated. Correlation with detailed tobacco history was then tested by statistical analysis. Results: All the non smoker OSCC cases showed positive immunoexpression. However 3 cases of the smoker patient revealed a weak positive immunostaining with only sporadic cells at the periphery. Conclusion: The results suggest that the absence of MGMT expression is related strongly to tobacco smoking and, thus, might be a significant event in oral carcinogenesis.},
 year = {2014}
}

Copy | Download

TY  - JOUR
T1  - Expression of O6 Methyl Guanine Methyl Transferase (Mgmt) in Oral Squamous Cell Carcinoma Patients in Alexandria, Egypt (Smokers and Non-Smokers)
AU  - Hend Mohamed Helmy
AU  - Taissir Ali Omar
AU  - Sahar Mohamed ElSheikh
AU  - Ahmed Serag Habib
Y1  - 2014/08/30
PY  - 2014
N1  - https://doi.org/10.11648/j.ajls.20140204.15
DO  - 10.11648/j.ajls.20140204.15
T2  - American Journal of Life Sciences
JF  - American Journal of Life Sciences
JO  - American Journal of Life Sciences
SP  - 217
EP  - 223
PB  - Science Publishing Group
SN  - 2328-5737
UR  - https://doi.org/10.11648/j.ajls.20140204.15
AB  - Objectives: O6-methylguanine-DNA methyltransferase (MGMT) removes mutagenic, carcinogenic, and cytotoxic adducts from O6-methylguanine in DNA through a direct reversal mechanism. Decreased expression of MGMT has been reported in a variety of human malignant tumors. The purpose of this study was to clarify the correlation of MGMT expression levels in oral squamous cell carcinoma (OSCC) and cigarette smoking. Study Design: MGMT protein expression in 22 cases of oral squamous cell carcinoma by immunohistochemistry was investigated. Correlation with detailed tobacco history was then tested by statistical analysis. Results: All the non smoker OSCC cases showed positive immunoexpression. However 3 cases of the smoker patient revealed a weak positive immunostaining with only sporadic cells at the periphery. Conclusion: The results suggest that the absence of MGMT expression is related strongly to tobacco smoking and, thus, might be a significant event in oral carcinogenesis.
VL  - 2
IS  - 4
ER  -

Copy | Download