Comparison of LPA1 and LPA2 Receptor Expression with Proliferative and Prognostic Factors in Endometroid Carcinomas and Endometrial Hyperplasias

Ayşegül Kaynar; Serdar Yanık; Ayşe Neslin Akkoca; Raziye Kurt; Ozan Turgut; Zeynep Tuba Özdemir; Nurdan Tatar; Ufuk Usta

doi:doi:10.11648/j.ajcem.20140204.12

Comparison of LPA1 and LPA2 Receptor Expression with Proliferative and Prognostic Factors in Endometroid Carcinomas and Endometrial Hyperplasias

Ayşegül Kaynar, Serdar Yanık, Ayşe Neslin Akkoca, Raziye Kurt, Ozan Turgut, Zeynep Tuba Özdemir, Nurdan Tatar, Ufuk Usta

Published in American Journal of Clinical and Experimental Medicine (Volume 2, Issue 4)

Received: 16 June 2014 Accepted: 1 July 2014 Published: 10 July 2014

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Abstract

Objective: In this study we aimed to evaluate the staining patterns of lysophosphatidic acid in endometrial carcinomas(EC) and endometrial hyperplasias(EH). Materials and Method: Sixty diagnostic cases were included in this study in order to evaluate the staining patterns of lysophosphatidic acid in EC and EH. EC was diagnosed in 20 of the cases, EH with atypia was present in 20 and EH without atypia was evident in 20 of the cases. Patients staged according to FİGO(International Federation of Gynecology and Obstetrics,2014). After performing the new sections on each of these cases, 55 routine Hematoxylin and eosin staining was repeated, sections of chosen diagnostic blocks were stained immune histologically with LPA1(Lysophosphatidic acid 1), LPA(Lysophosphatidic acid 2), MMP-2(Matrix metalloproteinase 2) and Ki-67 antibodies. Results: According to the data obtained, LPA1 showed most intense staining in cases with EH without atypia, however endometrioid type endometrial carcinoma (EEC) cases had the levels very close to this. Furthermore, it was found that there was a reverse correlation between LPA1 staining and histological grade in cases with EEC. It was noted that highest level of LPA2 staining was in cases that had EH with atypia where as lowest level was seen in cases with EEC cases. No relationship between LPA2 and the grade in cases with EEC. It was found that MMP-2 increased linearly with the histological grade in cases with EEC. Correlation tests done among LPA1, LPA2 and MMP-2 antibodies revealed moderate degree of relation only between LAP1 and MMP-2 scores in cases with EEC. No significant relation could be shown in correlation test done between LPA1 and LPA2 and Ki-67, a marker for proliferation index. Conclusion: When correlation of LPA1, LPA2 and MMP-2 H scores with stages of EEC cases was taken into account, the average LPA1 H score was higher in stage 1, while H score averages of LPA2 and MMP-2 H scores were higher in stage 2+3 tumors, however these differences were not statistically significant.

Published in	American Journal of Clinical and Experimental Medicine (Volume 2, Issue 4)
DOI	10.11648/j.ajcem.20140204.12
Page(s)	64-69
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2014. Published by Science Publishing Group

Keywords

LPA1-2, MMP-2 Immunohistochemistry, Endometrial Carcinoma, Endometrial Hyperplasia, FİGO, Endometrioid Carcinoma

References

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Ayşegül Kaynar, Serdar Yanık, Ayşe Neslin Akkoca, Raziye Kurt, Ozan Turgut, et al. (2014). Comparison of LPA1 and LPA2 Receptor Expression with Proliferative and Prognostic Factors in Endometroid Carcinomas and Endometrial Hyperplasias. American Journal of Clinical and Experimental Medicine, 2(4), 64-69. https://doi.org/10.11648/j.ajcem.20140204.12

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Ayşegül Kaynar; Serdar Yanık; Ayşe Neslin Akkoca; Raziye Kurt; Ozan Turgut, et al. Comparison of LPA1 and LPA2 Receptor Expression with Proliferative and Prognostic Factors in Endometroid Carcinomas and Endometrial Hyperplasias. Am. J. Clin. Exp. Med. 2014, 2(4), 64-69. doi: 10.11648/j.ajcem.20140204.12

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AMA Style

Ayşegül Kaynar, Serdar Yanık, Ayşe Neslin Akkoca, Raziye Kurt, Ozan Turgut, et al. Comparison of LPA1 and LPA2 Receptor Expression with Proliferative and Prognostic Factors in Endometroid Carcinomas and Endometrial Hyperplasias. Am J Clin Exp Med. 2014;2(4):64-69. doi: 10.11648/j.ajcem.20140204.12

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@article{10.11648/j.ajcem.20140204.12,
  author = {Ayşegül Kaynar and Serdar Yanık and Ayşe Neslin Akkoca and Raziye Kurt and Ozan Turgut and Zeynep Tuba Özdemir and Nurdan Tatar and Ufuk Usta},
  title = {Comparison of LPA1 and LPA2 Receptor Expression with Proliferative and Prognostic Factors in Endometroid Carcinomas and Endometrial Hyperplasias},
  journal = {American Journal of Clinical and Experimental Medicine},
  volume = {2},
  number = {4},
  pages = {64-69},
  doi = {10.11648/j.ajcem.20140204.12},
  url = {https://doi.org/10.11648/j.ajcem.20140204.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcem.20140204.12},
  abstract = {Objective: In this study we aimed to evaluate the staining patterns of lysophosphatidic acid in endometrial carcinomas(EC) and endometrial hyperplasias(EH). Materials and Method: Sixty diagnostic cases were included in this study in order to evaluate the staining patterns of lysophosphatidic acid in EC and EH. EC was diagnosed in 20 of the cases, EH with atypia was present in 20 and EH without atypia was evident in 20 of the cases. Patients staged according to FİGO(International Federation of Gynecology and Obstetrics,2014). After performing the new sections on each of these cases, 55 routine Hematoxylin and eosin staining was repeated, sections of chosen diagnostic blocks were stained immune histologically with  LPA1(Lysophosphatidic acid 1), LPA(Lysophosphatidic acid 2), MMP-2(Matrix metalloproteinase 2) and Ki-67 antibodies. Results: According to the data obtained, LPA1 showed most intense staining in cases with EH without atypia, however endometrioid type endometrial carcinoma (EEC) cases had the levels very close to this. Furthermore, it was found that there was a reverse correlation between LPA1 staining and histological grade in cases with EEC. It was noted that highest level of LPA2 staining was in cases that had EH with atypia where as lowest level was seen in cases with EEC cases. No relationship between LPA2 and the grade in cases with EEC. It was found that MMP-2 increased linearly with the histological grade in cases with EEC. Correlation tests done among LPA1, LPA2 and MMP-2 antibodies revealed moderate degree of relation only between LAP1 and MMP-2 scores in cases with EEC. No significant relation could be shown in correlation test done between LPA1 and LPA2 and Ki-67, a marker for proliferation index. Conclusion: When correlation of LPA1, LPA2 and MMP-2 H scores with stages of EEC cases was taken into account, the average LPA1 H score was higher in stage 1, while H score averages of LPA2 and MMP-2 H scores were higher in stage 2+3 tumors, however these differences were not statistically significant.},
 year = {2014}
}

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TY  - JOUR
T1  - Comparison of LPA1 and LPA2 Receptor Expression with Proliferative and Prognostic Factors in Endometroid Carcinomas and Endometrial Hyperplasias
AU  - Ayşegül Kaynar
AU  - Serdar Yanık
AU  - Ayşe Neslin Akkoca
AU  - Raziye Kurt
AU  - Ozan Turgut
AU  - Zeynep Tuba Özdemir
AU  - Nurdan Tatar
AU  - Ufuk Usta
Y1  - 2014/07/10
PY  - 2014
N1  - https://doi.org/10.11648/j.ajcem.20140204.12
DO  - 10.11648/j.ajcem.20140204.12
T2  - American Journal of Clinical and Experimental Medicine
JF  - American Journal of Clinical and Experimental Medicine
JO  - American Journal of Clinical and Experimental Medicine
SP  - 64
EP  - 69
PB  - Science Publishing Group
SN  - 2330-8133
UR  - https://doi.org/10.11648/j.ajcem.20140204.12
AB  - Objective: In this study we aimed to evaluate the staining patterns of lysophosphatidic acid in endometrial carcinomas(EC) and endometrial hyperplasias(EH). Materials and Method: Sixty diagnostic cases were included in this study in order to evaluate the staining patterns of lysophosphatidic acid in EC and EH. EC was diagnosed in 20 of the cases, EH with atypia was present in 20 and EH without atypia was evident in 20 of the cases. Patients staged according to FİGO(International Federation of Gynecology and Obstetrics,2014). After performing the new sections on each of these cases, 55 routine Hematoxylin and eosin staining was repeated, sections of chosen diagnostic blocks were stained immune histologically with  LPA1(Lysophosphatidic acid 1), LPA(Lysophosphatidic acid 2), MMP-2(Matrix metalloproteinase 2) and Ki-67 antibodies. Results: According to the data obtained, LPA1 showed most intense staining in cases with EH without atypia, however endometrioid type endometrial carcinoma (EEC) cases had the levels very close to this. Furthermore, it was found that there was a reverse correlation between LPA1 staining and histological grade in cases with EEC. It was noted that highest level of LPA2 staining was in cases that had EH with atypia where as lowest level was seen in cases with EEC cases. No relationship between LPA2 and the grade in cases with EEC. It was found that MMP-2 increased linearly with the histological grade in cases with EEC. Correlation tests done among LPA1, LPA2 and MMP-2 antibodies revealed moderate degree of relation only between LAP1 and MMP-2 scores in cases with EEC. No significant relation could be shown in correlation test done between LPA1 and LPA2 and Ki-67, a marker for proliferation index. Conclusion: When correlation of LPA1, LPA2 and MMP-2 H scores with stages of EEC cases was taken into account, the average LPA1 H score was higher in stage 1, while H score averages of LPA2 and MMP-2 H scores were higher in stage 2+3 tumors, however these differences were not statistically significant.
VL  - 2
IS  - 4
ER  -

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Author Information

Ayşegül Kaynar
Serdar Yanık
Ayşe Neslin Akkoca
Raziye Kurt

Department of Obstetrics and Gynecology, Mustafa Kemal University, Medical School, Hatay, Turkey
Ozan Turgut

Department of Obstetrics and Gynecology, ?skenderun State Hospital, Hatay, Turkey
Zeynep Tuba Özdemir
Nurdan Tatar

Department of Patology, ?skenderun State Hospital, Hatay, Turkey
Ufuk Usta

Department of Patology, Trakya University Medical School, Edirne, Turkey

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APA Style

Ayşegül Kaynar, Serdar Yanık, Ayşe Neslin Akkoca, Raziye Kurt, Ozan Turgut, et al. (2014). Comparison of LPA1 and LPA2 Receptor Expression with Proliferative and Prognostic Factors in Endometroid Carcinomas and Endometrial Hyperplasias. American Journal of Clinical and Experimental Medicine, 2(4), 64-69. https://doi.org/10.11648/j.ajcem.20140204.12

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ACS Style

Ayşegül Kaynar; Serdar Yanık; Ayşe Neslin Akkoca; Raziye Kurt; Ozan Turgut, et al. Comparison of LPA1 and LPA2 Receptor Expression with Proliferative and Prognostic Factors in Endometroid Carcinomas and Endometrial Hyperplasias. Am. J. Clin. Exp. Med. 2014, 2(4), 64-69. doi: 10.11648/j.ajcem.20140204.12

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AMA Style

Ayşegül Kaynar, Serdar Yanık, Ayşe Neslin Akkoca, Raziye Kurt, Ozan Turgut, et al. Comparison of LPA1 and LPA2 Receptor Expression with Proliferative and Prognostic Factors in Endometroid Carcinomas and Endometrial Hyperplasias. Am J Clin Exp Med. 2014;2(4):64-69. doi: 10.11648/j.ajcem.20140204.12

Copy | Download

@article{10.11648/j.ajcem.20140204.12,
  author = {Ayşegül Kaynar and Serdar Yanık and Ayşe Neslin Akkoca and Raziye Kurt and Ozan Turgut and Zeynep Tuba Özdemir and Nurdan Tatar and Ufuk Usta},
  title = {Comparison of LPA1 and LPA2 Receptor Expression with Proliferative and Prognostic Factors in Endometroid Carcinomas and Endometrial Hyperplasias},
  journal = {American Journal of Clinical and Experimental Medicine},
  volume = {2},
  number = {4},
  pages = {64-69},
  doi = {10.11648/j.ajcem.20140204.12},
  url = {https://doi.org/10.11648/j.ajcem.20140204.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcem.20140204.12},
  abstract = {Objective: In this study we aimed to evaluate the staining patterns of lysophosphatidic acid in endometrial carcinomas(EC) and endometrial hyperplasias(EH). Materials and Method: Sixty diagnostic cases were included in this study in order to evaluate the staining patterns of lysophosphatidic acid in EC and EH. EC was diagnosed in 20 of the cases, EH with atypia was present in 20 and EH without atypia was evident in 20 of the cases. Patients staged according to FİGO(International Federation of Gynecology and Obstetrics,2014). After performing the new sections on each of these cases, 55 routine Hematoxylin and eosin staining was repeated, sections of chosen diagnostic blocks were stained immune histologically with  LPA1(Lysophosphatidic acid 1), LPA(Lysophosphatidic acid 2), MMP-2(Matrix metalloproteinase 2) and Ki-67 antibodies. Results: According to the data obtained, LPA1 showed most intense staining in cases with EH without atypia, however endometrioid type endometrial carcinoma (EEC) cases had the levels very close to this. Furthermore, it was found that there was a reverse correlation between LPA1 staining and histological grade in cases with EEC. It was noted that highest level of LPA2 staining was in cases that had EH with atypia where as lowest level was seen in cases with EEC cases. No relationship between LPA2 and the grade in cases with EEC. It was found that MMP-2 increased linearly with the histological grade in cases with EEC. Correlation tests done among LPA1, LPA2 and MMP-2 antibodies revealed moderate degree of relation only between LAP1 and MMP-2 scores in cases with EEC. No significant relation could be shown in correlation test done between LPA1 and LPA2 and Ki-67, a marker for proliferation index. Conclusion: When correlation of LPA1, LPA2 and MMP-2 H scores with stages of EEC cases was taken into account, the average LPA1 H score was higher in stage 1, while H score averages of LPA2 and MMP-2 H scores were higher in stage 2+3 tumors, however these differences were not statistically significant.},
 year = {2014}
}

Copy | Download

TY  - JOUR
T1  - Comparison of LPA1 and LPA2 Receptor Expression with Proliferative and Prognostic Factors in Endometroid Carcinomas and Endometrial Hyperplasias
AU  - Ayşegül Kaynar
AU  - Serdar Yanık
AU  - Ayşe Neslin Akkoca
AU  - Raziye Kurt
AU  - Ozan Turgut
AU  - Zeynep Tuba Özdemir
AU  - Nurdan Tatar
AU  - Ufuk Usta
Y1  - 2014/07/10
PY  - 2014
N1  - https://doi.org/10.11648/j.ajcem.20140204.12
DO  - 10.11648/j.ajcem.20140204.12
T2  - American Journal of Clinical and Experimental Medicine
JF  - American Journal of Clinical and Experimental Medicine
JO  - American Journal of Clinical and Experimental Medicine
SP  - 64
EP  - 69
PB  - Science Publishing Group
SN  - 2330-8133
UR  - https://doi.org/10.11648/j.ajcem.20140204.12
AB  - Objective: In this study we aimed to evaluate the staining patterns of lysophosphatidic acid in endometrial carcinomas(EC) and endometrial hyperplasias(EH). Materials and Method: Sixty diagnostic cases were included in this study in order to evaluate the staining patterns of lysophosphatidic acid in EC and EH. EC was diagnosed in 20 of the cases, EH with atypia was present in 20 and EH without atypia was evident in 20 of the cases. Patients staged according to FİGO(International Federation of Gynecology and Obstetrics,2014). After performing the new sections on each of these cases, 55 routine Hematoxylin and eosin staining was repeated, sections of chosen diagnostic blocks were stained immune histologically with  LPA1(Lysophosphatidic acid 1), LPA(Lysophosphatidic acid 2), MMP-2(Matrix metalloproteinase 2) and Ki-67 antibodies. Results: According to the data obtained, LPA1 showed most intense staining in cases with EH without atypia, however endometrioid type endometrial carcinoma (EEC) cases had the levels very close to this. Furthermore, it was found that there was a reverse correlation between LPA1 staining and histological grade in cases with EEC. It was noted that highest level of LPA2 staining was in cases that had EH with atypia where as lowest level was seen in cases with EEC cases. No relationship between LPA2 and the grade in cases with EEC. It was found that MMP-2 increased linearly with the histological grade in cases with EEC. Correlation tests done among LPA1, LPA2 and MMP-2 antibodies revealed moderate degree of relation only between LAP1 and MMP-2 scores in cases with EEC. No significant relation could be shown in correlation test done between LPA1 and LPA2 and Ki-67, a marker for proliferation index. Conclusion: When correlation of LPA1, LPA2 and MMP-2 H scores with stages of EEC cases was taken into account, the average LPA1 H score was higher in stage 1, while H score averages of LPA2 and MMP-2 H scores were higher in stage 2+3 tumors, however these differences were not statistically significant.
VL  - 2
IS  - 4
ER  -

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