Experimental Investigation on the Ice Formation and Growth in Ex Vivo Bovine Liver

Valter Giaretto; Claudio Passerone

doi:doi:10.11648/j.ajbio.20180603.11

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Experimental Investigation on the Ice Formation and Growth in Ex Vivo Bovine Liver

Valter Giaretto, Claudio Passerone

Published in American Journal of BioScience (Volume 6, Issue 3)

Received: 20 September 2018 Accepted: 11 October 2018 Published: 15 November 2018

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Abstract

The paper presents a set of experiments to characterize the ice front propagation in ex vivo bovine liver samples. Based on previous successful experiments using water and agar-gel as tested materials, the methodology infers the ice evolution in the sample from temperature measures obtained at the cooling device only, with a technique known as mirror image. No analytical or numerical solutions are proposed for the phase change propagation inside the tested material, such as for instance the Stefan approach as a classical free boundary problem for a parabolic partial differential equation. Thermal events inside the tested material, such as the heat flux released during the phase transition, have been deduced by the measured temperatures and the numerical solution of the thermal field inside the cooling device, conceived to mimic some thermal features of an actual cryogenic probe. The application domain for these experiments is in the cryoablative therapy, and the aim is to help providing an increased knowledge on the thermal effects to better deduce the effectiveness of the treatment and reduce recurrences, while at the same time avoid damages in the proximal anatomical structures. Results show that it is possible to detect the triggering of ice formation at the interface between the liver samples and the cooling device and predict the ice front propagation according to a law linear on the heat subtracted per unit of area. In a few tens of seconds, the maximum ice penetration distance is about 2 mm inside the liver tissue, with a penetration rate that goes from 0.2 mm/s to 0.02 mm/s. Moreover, adopting a special sample configured as an agar-gel coating superimposed to an ex vivo liver layer, the arrival time of the ice penetrating the liver and the temperature at the interface between these materials were detected, in order to estimate the part of the heat flux useful to the ice formation with respect to that spent for cooling the surrounding medium. Based on this preliminary result, to improve the cryoablation effectiveness it could be useful to increase the heat flux per unit of surface at the beginning, instead of the ablation duration.

Published in	American Journal of BioScience (Volume 6, Issue 3)
DOI	10.11648/j.ajbio.20180603.11
Page(s)	35-44
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2018. Published by Science Publishing Group

Keywords

Cryoablation, Ex Vivo Liver, Mirror Image Technique, Ice formation in Tissue, Cryosurgery

References

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APA Style

Valter Giaretto, Claudio Passerone. (2018). Experimental Investigation on the Ice Formation and Growth in Ex Vivo Bovine Liver. American Journal of BioScience, 6(3), 35-44. https://doi.org/10.11648/j.ajbio.20180603.11

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ACS Style

Valter Giaretto; Claudio Passerone. Experimental Investigation on the Ice Formation and Growth in Ex Vivo Bovine Liver. Am. J. BioScience 2018, 6(3), 35-44. doi: 10.11648/j.ajbio.20180603.11

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AMA Style

Valter Giaretto, Claudio Passerone. Experimental Investigation on the Ice Formation and Growth in Ex Vivo Bovine Liver. Am J BioScience. 2018;6(3):35-44. doi: 10.11648/j.ajbio.20180603.11

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@article{10.11648/j.ajbio.20180603.11,
  author = {Valter Giaretto and Claudio Passerone},
  title = {Experimental Investigation on the Ice Formation and Growth in Ex Vivo Bovine Liver},
  journal = {American Journal of BioScience},
  volume = {6},
  number = {3},
  pages = {35-44},
  doi = {10.11648/j.ajbio.20180603.11},
  url = {https://doi.org/10.11648/j.ajbio.20180603.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.20180603.11},
  abstract = {The paper presents a set of experiments to characterize the ice front propagation in ex vivo bovine liver samples. Based on previous successful experiments using water and agar-gel as tested materials, the methodology infers the ice evolution in the sample from temperature measures obtained at the cooling device only, with a technique known as mirror image. No analytical or numerical solutions are proposed for the phase change propagation inside the tested material, such as for instance the Stefan approach as a classical free boundary problem for a parabolic partial differential equation. Thermal events inside the tested material, such as the heat flux released during the phase transition, have been deduced by the measured temperatures and the numerical solution of the thermal field inside the cooling device, conceived to mimic some thermal features of an actual cryogenic probe. The application domain for these experiments is in the cryoablative therapy, and the aim is to help providing an increased knowledge on the thermal effects to better deduce the effectiveness of the treatment and reduce recurrences, while at the same time avoid damages in the proximal anatomical structures. Results show that it is possible to detect the triggering of ice formation at the interface between the liver samples and the cooling device and predict the ice front propagation according to a law linear on the heat subtracted per unit of area. In a few tens of seconds, the maximum ice penetration distance is about 2 mm inside the liver tissue, with a penetration rate that goes from 0.2 mm/s to 0.02 mm/s. Moreover, adopting a special sample configured as an agar-gel coating superimposed to an ex vivo liver layer, the arrival time of the ice penetrating the liver and the temperature at the interface between these materials were detected, in order to estimate the part of the heat flux useful to the ice formation with respect to that spent for cooling the surrounding medium. Based on this preliminary result, to improve the cryoablation effectiveness it could be useful to increase the heat flux per unit of surface at the beginning, instead of the ablation duration.},
 year = {2018}
}

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TY  - JOUR
T1  - Experimental Investigation on the Ice Formation and Growth in Ex Vivo Bovine Liver
AU  - Valter Giaretto
AU  - Claudio Passerone
Y1  - 2018/11/15
PY  - 2018
N1  - https://doi.org/10.11648/j.ajbio.20180603.11
DO  - 10.11648/j.ajbio.20180603.11
T2  - American Journal of BioScience
JF  - American Journal of BioScience
JO  - American Journal of BioScience
SP  - 35
EP  - 44
PB  - Science Publishing Group
SN  - 2330-0167
UR  - https://doi.org/10.11648/j.ajbio.20180603.11
AB  - The paper presents a set of experiments to characterize the ice front propagation in ex vivo bovine liver samples. Based on previous successful experiments using water and agar-gel as tested materials, the methodology infers the ice evolution in the sample from temperature measures obtained at the cooling device only, with a technique known as mirror image. No analytical or numerical solutions are proposed for the phase change propagation inside the tested material, such as for instance the Stefan approach as a classical free boundary problem for a parabolic partial differential equation. Thermal events inside the tested material, such as the heat flux released during the phase transition, have been deduced by the measured temperatures and the numerical solution of the thermal field inside the cooling device, conceived to mimic some thermal features of an actual cryogenic probe. The application domain for these experiments is in the cryoablative therapy, and the aim is to help providing an increased knowledge on the thermal effects to better deduce the effectiveness of the treatment and reduce recurrences, while at the same time avoid damages in the proximal anatomical structures. Results show that it is possible to detect the triggering of ice formation at the interface between the liver samples and the cooling device and predict the ice front propagation according to a law linear on the heat subtracted per unit of area. In a few tens of seconds, the maximum ice penetration distance is about 2 mm inside the liver tissue, with a penetration rate that goes from 0.2 mm/s to 0.02 mm/s. Moreover, adopting a special sample configured as an agar-gel coating superimposed to an ex vivo liver layer, the arrival time of the ice penetrating the liver and the temperature at the interface between these materials were detected, in order to estimate the part of the heat flux useful to the ice formation with respect to that spent for cooling the surrounding medium. Based on this preliminary result, to improve the cryoablation effectiveness it could be useful to increase the heat flux per unit of surface at the beginning, instead of the ablation duration.
VL  - 6
IS  - 3
ER  -

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Author Information

Valter Giaretto

Department of Energy, Politecnico di Torino, Torino, Italy
Claudio Passerone

Department of Electronics and Telecommunications, Politecnico di Torino, Torino, Italy

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APA Style

Valter Giaretto, Claudio Passerone. (2018). Experimental Investigation on the Ice Formation and Growth in Ex Vivo Bovine Liver. American Journal of BioScience, 6(3), 35-44. https://doi.org/10.11648/j.ajbio.20180603.11

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ACS Style

Valter Giaretto; Claudio Passerone. Experimental Investigation on the Ice Formation and Growth in Ex Vivo Bovine Liver. Am. J. BioScience 2018, 6(3), 35-44. doi: 10.11648/j.ajbio.20180603.11

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AMA Style

Valter Giaretto, Claudio Passerone. Experimental Investigation on the Ice Formation and Growth in Ex Vivo Bovine Liver. Am J BioScience. 2018;6(3):35-44. doi: 10.11648/j.ajbio.20180603.11

Copy | Download

@article{10.11648/j.ajbio.20180603.11,
  author = {Valter Giaretto and Claudio Passerone},
  title = {Experimental Investigation on the Ice Formation and Growth in Ex Vivo Bovine Liver},
  journal = {American Journal of BioScience},
  volume = {6},
  number = {3},
  pages = {35-44},
  doi = {10.11648/j.ajbio.20180603.11},
  url = {https://doi.org/10.11648/j.ajbio.20180603.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.20180603.11},
  abstract = {The paper presents a set of experiments to characterize the ice front propagation in ex vivo bovine liver samples. Based on previous successful experiments using water and agar-gel as tested materials, the methodology infers the ice evolution in the sample from temperature measures obtained at the cooling device only, with a technique known as mirror image. No analytical or numerical solutions are proposed for the phase change propagation inside the tested material, such as for instance the Stefan approach as a classical free boundary problem for a parabolic partial differential equation. Thermal events inside the tested material, such as the heat flux released during the phase transition, have been deduced by the measured temperatures and the numerical solution of the thermal field inside the cooling device, conceived to mimic some thermal features of an actual cryogenic probe. The application domain for these experiments is in the cryoablative therapy, and the aim is to help providing an increased knowledge on the thermal effects to better deduce the effectiveness of the treatment and reduce recurrences, while at the same time avoid damages in the proximal anatomical structures. Results show that it is possible to detect the triggering of ice formation at the interface between the liver samples and the cooling device and predict the ice front propagation according to a law linear on the heat subtracted per unit of area. In a few tens of seconds, the maximum ice penetration distance is about 2 mm inside the liver tissue, with a penetration rate that goes from 0.2 mm/s to 0.02 mm/s. Moreover, adopting a special sample configured as an agar-gel coating superimposed to an ex vivo liver layer, the arrival time of the ice penetrating the liver and the temperature at the interface between these materials were detected, in order to estimate the part of the heat flux useful to the ice formation with respect to that spent for cooling the surrounding medium. Based on this preliminary result, to improve the cryoablation effectiveness it could be useful to increase the heat flux per unit of surface at the beginning, instead of the ablation duration.},
 year = {2018}
}

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TY  - JOUR
T1  - Experimental Investigation on the Ice Formation and Growth in Ex Vivo Bovine Liver
AU  - Valter Giaretto
AU  - Claudio Passerone
Y1  - 2018/11/15
PY  - 2018
N1  - https://doi.org/10.11648/j.ajbio.20180603.11
DO  - 10.11648/j.ajbio.20180603.11
T2  - American Journal of BioScience
JF  - American Journal of BioScience
JO  - American Journal of BioScience
SP  - 35
EP  - 44
PB  - Science Publishing Group
SN  - 2330-0167
UR  - https://doi.org/10.11648/j.ajbio.20180603.11
AB  - The paper presents a set of experiments to characterize the ice front propagation in ex vivo bovine liver samples. Based on previous successful experiments using water and agar-gel as tested materials, the methodology infers the ice evolution in the sample from temperature measures obtained at the cooling device only, with a technique known as mirror image. No analytical or numerical solutions are proposed for the phase change propagation inside the tested material, such as for instance the Stefan approach as a classical free boundary problem for a parabolic partial differential equation. Thermal events inside the tested material, such as the heat flux released during the phase transition, have been deduced by the measured temperatures and the numerical solution of the thermal field inside the cooling device, conceived to mimic some thermal features of an actual cryogenic probe. The application domain for these experiments is in the cryoablative therapy, and the aim is to help providing an increased knowledge on the thermal effects to better deduce the effectiveness of the treatment and reduce recurrences, while at the same time avoid damages in the proximal anatomical structures. Results show that it is possible to detect the triggering of ice formation at the interface between the liver samples and the cooling device and predict the ice front propagation according to a law linear on the heat subtracted per unit of area. In a few tens of seconds, the maximum ice penetration distance is about 2 mm inside the liver tissue, with a penetration rate that goes from 0.2 mm/s to 0.02 mm/s. Moreover, adopting a special sample configured as an agar-gel coating superimposed to an ex vivo liver layer, the arrival time of the ice penetrating the liver and the temperature at the interface between these materials were detected, in order to estimate the part of the heat flux useful to the ice formation with respect to that spent for cooling the surrounding medium. Based on this preliminary result, to improve the cryoablation effectiveness it could be useful to increase the heat flux per unit of surface at the beginning, instead of the ablation duration.
VL  - 6
IS  - 3
ER  -

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