Molecular Characterization and Phylogenetic Analysis of Growth Hormone cDNA Sequence from the Acipenser Persicus

E. Nasr; H. G. Hovhannisyan; M. Pourkazemi; L. Azizzadeh

doi:doi:10.11648/j.ajbio.20140202.20

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Molecular Characterization and Phylogenetic Analysis of Growth Hormone cDNA Sequence from the Acipenser Persicus

E. Nasr, H. G. Hovhannisyan, M. Pourkazemi, L. Azizzadeh

Published in American Journal of BioScience (Volume 2, Issue 2)

Received: 6 March 2014 Accepted: 11 April 2014 Published: 20 April 2014

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Abstract

The aim of this study was to investigate the amount of variation between Acipenseridea family and also, study amount of phylogenetic variation between Persian sturgeon (Acipenser persicus) with other Acipenseridea regarding to the Growth Hormon (GH) gene. In this study, pre growth hormone gene of Persian sturgeon was identified for first time and applied to gene bank (JN604534.1, 2011). The total RNA was extracted from pituitary gland of Persian Sturgeon, cDNA was synthesized. The full-length cDNA sequence of Persian sturgeon contains a 645 nucleotide open reading frame, which encodes a peptide of 214 amino acids. The position of the signal peptide cleavage site was predicted to be at position 72. After cleaving of a signal peptide of 24 amino acid resi¬dues and a mature peptide of 190 aa formed. Multiple sequence alignments and Phylogenic tree were performed by using the MEGA5 program. By sequence alignment and phylogenetic analysis of amino acid residues indicated that GH widely conserved in other species were identified. The GH nucleic acid and amino acid residue sequences of Persian sturgeon had a highest similarity to these of other Acipenseridea as well as mammalian, followed by those of Anguiliformes

Published in	American Journal of BioScience (Volume 2, Issue 2)
DOI	10.11648/j.ajbio.20140202.20
Page(s)	79-83
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2014. Published by Science Publishing Group

Keywords

Persian Sturgeon, Growth Hormone, cDNA, Phylogenetic Analysis

References

[1]	Chang YS, Liu CS, Huang FL, Lo TB, 1992.The primary structures of growth hormones of three cyprinid species, bighead carp, common carp and grass carp, gen comp endocrinal. 87, 385-93
[2]	IUCN (International Union for Conservation of Nature). 2010. Acipenser persicus. Available at www.iucnredlist.org/apps/redlist/details/235/0 [viewed 12/7/11].
[3]	Jiang, B.C, et al, 2012. Doxycycline-regulated growth hormone gene expression system for swine. Genetics and Molecular Research 11 (3): 2946-2957
[4]	Khoshkholgh M, et al, 2011.Genetic diversity in the Persian sturgeon, Acipenser percicus, from the south , Caspian J. Env. Sci. Vol. 9 No.1 pp. 17~25
[5]	Luis F. Marins et al. A growth hormone-based phylogenetic analysis of euteleostean fishes including a representative species of the Atheriniformes Order, Odontesthes argentinensis. 2003. Genetics and Molecular Biology, 26, 3, 295-300
[6]	Paul, K. Persian Sturgeon (Acipenser persicus) in Pond Life. 2007-2011 edition. World Wide Web electronic publication. Available at www.pondlife. me.uk/sturgeon/acipenserpersicus.php [viewed 12/8/11].
[7]	Rousseau.K and Dufour. S. 2004. Phylogenetic evolution of the neuroendocrine control of growth hormone: Contribution from Teleosts. Cybium, 28(3): 181-198.
[8]	Vajhi. A.R. et al; 2008 Digestive System Ultrasonography in Acipenser persicus Proceedings, The 15th Congress of FAVA. FAVA - OIE Joint Symposium on Emerging Diseases in Bangkok, Thailand on 27-30 October2008.page145-146
[9]	Venugopal T, et al. 2002.Molecular cloning of growth hormone encoding cDNA of Indian major carps by a modified rapid amplification of cDNA ends strategy. J. Biosci. Indian Academy of Sciences ( Vol. 27 , No. 3, ( 261–272 )
[10]	Vestling M, Murphy C, Fenselau C, Chen TT, 199.1Disulfide bonds in native and recombinant fish growth hormone. Mol Mar Biol Biotechnology, 1.73-7.

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APA Style

E. Nasr, H. G. Hovhannisyan, M. Pourkazemi, L. Azizzadeh. (2014). Molecular Characterization and Phylogenetic Analysis of Growth Hormone cDNA Sequence from the Acipenser Persicus. American Journal of BioScience, 2(2), 79-83. https://doi.org/10.11648/j.ajbio.20140202.20

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ACS Style

E. Nasr; H. G. Hovhannisyan; M. Pourkazemi; L. Azizzadeh. Molecular Characterization and Phylogenetic Analysis of Growth Hormone cDNA Sequence from the Acipenser Persicus. Am. J. BioScience 2014, 2(2), 79-83. doi: 10.11648/j.ajbio.20140202.20

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AMA Style

E. Nasr, H. G. Hovhannisyan, M. Pourkazemi, L. Azizzadeh. Molecular Characterization and Phylogenetic Analysis of Growth Hormone cDNA Sequence from the Acipenser Persicus. Am J BioScience. 2014;2(2):79-83. doi: 10.11648/j.ajbio.20140202.20

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@article{10.11648/j.ajbio.20140202.20,
  author = {E. Nasr and H. G. Hovhannisyan and M. Pourkazemi and L. Azizzadeh},
  title = {Molecular Characterization and Phylogenetic Analysis of Growth Hormone cDNA Sequence from the Acipenser Persicus},
  journal = {American Journal of BioScience},
  volume = {2},
  number = {2},
  pages = {79-83},
  doi = {10.11648/j.ajbio.20140202.20},
  url = {https://doi.org/10.11648/j.ajbio.20140202.20},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.20140202.20},
  abstract = {The aim of this study was to investigate the amount of variation between Acipenseridea family and also, study amount of phylogenetic variation between Persian sturgeon (Acipenser persicus) with other Acipenseridea regarding to the Growth Hormon (GH) gene. In this study, pre growth hormone gene of Persian sturgeon was identified for first time and applied to gene bank (JN604534.1, 2011). The total RNA was extracted from pituitary gland of Persian Sturgeon, cDNA was synthesized. The full-length cDNA sequence of Persian sturgeon contains a 645 nucleotide open reading frame, which encodes a peptide of 214 amino acids. The position of the signal peptide cleavage site was predicted to be at position 72. After cleaving of a signal peptide of 24 amino acid resi¬dues and a mature peptide of 190 aa formed. Multiple sequence alignments and Phylogenic tree were performed by using the MEGA5 program. By sequence alignment and phylogenetic analysis of amino acid residues indicated that GH widely conserved in other species were identified. The GH nucleic acid and amino acid residue sequences of Persian sturgeon had a highest similarity to these of other Acipenseridea as well as mammalian, followed by those of Anguiliformes},
 year = {2014}
}

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TY - JOUR
T1 - Molecular Characterization and Phylogenetic Analysis of Growth Hormone cDNA Sequence from the Acipenser Persicus
AU - E. Nasr
AU - H. G. Hovhannisyan
AU - M. Pourkazemi
AU - L. Azizzadeh
Y1 - 2014/04/20
PY - 2014
N1 - https://doi.org/10.11648/j.ajbio.20140202.20
DO - 10.11648/j.ajbio.20140202.20
T2 - American Journal of BioScience
JF - American Journal of BioScience
JO - American Journal of BioScience
SP - 79
EP - 83
PB - Science Publishing Group
SN - 2330-0167
UR - https://doi.org/10.11648/j.ajbio.20140202.20
AB - The aim of this study was to investigate the amount of variation between Acipenseridea family and also, study amount of phylogenetic variation between Persian sturgeon (Acipenser persicus) with other Acipenseridea regarding to the Growth Hormon (GH) gene. In this study, pre growth hormone gene of Persian sturgeon was identified for first time and applied to gene bank (JN604534.1, 2011). The total RNA was extracted from pituitary gland of Persian Sturgeon, cDNA was synthesized. The full-length cDNA sequence of Persian sturgeon contains a 645 nucleotide open reading frame, which encodes a peptide of 214 amino acids. The position of the signal peptide cleavage site was predicted to be at position 72. After cleaving of a signal peptide of 24 amino acid resi¬dues and a mature peptide of 190 aa formed. Multiple sequence alignments and Phylogenic tree were performed by using the MEGA5 program. By sequence alignment and phylogenetic analysis of amino acid residues indicated that GH widely conserved in other species were identified. The GH nucleic acid and amino acid residue sequences of Persian sturgeon had a highest similarity to these of other Acipenseridea as well as mammalian, followed by those of Anguiliformes
VL - 2
IS - 2
ER -

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Author Information

E. Nasr

Islamic Azad University, Meshgin Shahr Iran
H. G. Hovhannisyan

Scientific and Production Center “Armbiotechnology”, National Academy of Sciences of Armenia, Yerevan, Armenia
M. Pourkazemi

International sturgeon Research institute, Rasht, Iran
L. Azizzadeh

International sturgeon Research institute, Rasht, Iran

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APA Style

E. Nasr, H. G. Hovhannisyan, M. Pourkazemi, L. Azizzadeh. (2014). Molecular Characterization and Phylogenetic Analysis of Growth Hormone cDNA Sequence from the Acipenser Persicus. American Journal of BioScience, 2(2), 79-83. https://doi.org/10.11648/j.ajbio.20140202.20

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ACS Style

E. Nasr; H. G. Hovhannisyan; M. Pourkazemi; L. Azizzadeh. Molecular Characterization and Phylogenetic Analysis of Growth Hormone cDNA Sequence from the Acipenser Persicus. Am. J. BioScience 2014, 2(2), 79-83. doi: 10.11648/j.ajbio.20140202.20

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AMA Style

E. Nasr, H. G. Hovhannisyan, M. Pourkazemi, L. Azizzadeh. Molecular Characterization and Phylogenetic Analysis of Growth Hormone cDNA Sequence from the Acipenser Persicus. Am J BioScience. 2014;2(2):79-83. doi: 10.11648/j.ajbio.20140202.20

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@article{10.11648/j.ajbio.20140202.20,
  author = {E. Nasr and H. G. Hovhannisyan and M. Pourkazemi and L. Azizzadeh},
  title = {Molecular Characterization and Phylogenetic Analysis of Growth Hormone cDNA Sequence from the Acipenser Persicus},
  journal = {American Journal of BioScience},
  volume = {2},
  number = {2},
  pages = {79-83},
  doi = {10.11648/j.ajbio.20140202.20},
  url = {https://doi.org/10.11648/j.ajbio.20140202.20},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.20140202.20},
  abstract = {The aim of this study was to investigate the amount of variation between Acipenseridea family and also, study amount of phylogenetic variation between Persian sturgeon (Acipenser persicus) with other Acipenseridea regarding to the Growth Hormon (GH) gene. In this study, pre growth hormone gene of Persian sturgeon was identified for first time and applied to gene bank (JN604534.1, 2011). The total RNA was extracted from pituitary gland of Persian Sturgeon, cDNA was synthesized. The full-length cDNA sequence of Persian sturgeon contains a 645 nucleotide open reading frame, which encodes a peptide of 214 amino acids. The position of the signal peptide cleavage site was predicted to be at position 72. After cleaving of a signal peptide of 24 amino acid resi¬dues and a mature peptide of 190 aa formed. Multiple sequence alignments and Phylogenic tree were performed by using the MEGA5 program. By sequence alignment and phylogenetic analysis of amino acid residues indicated that GH widely conserved in other species were identified. The GH nucleic acid and amino acid residue sequences of Persian sturgeon had a highest similarity to these of other Acipenseridea as well as mammalian, followed by those of Anguiliformes},
 year = {2014}
}

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